APPENDIX.
Primer sequences for microsatellites isolated from BACs
| Locus | BACa | Forward primer | Reverse primer |
|---|---|---|---|
| OMM3000 | B10 | GAGGTGTGGAAGGGGAATAGG | AAAGATGTTGGGCTTGGCA |
| OMM3001 | C1 | AAATGGATGATGACTGTACTA | CACACATCTCTTTGTGACA |
| OMM 3012 | B8 | TTCTCCAGGTCCTACTCCAAGT | TTTTGGAGATGAGGTGAGGG |
| OMM 3018 | E1 | CATTGGGCCCTGAGTACAGT | CACCTCTGCCAATCTAGCAA |
| OMM 3020 | F1 | CGGACACCCTGACAAGATAAC | GACAGGGACGTGACAGTGAA |
| OMM 3032 | 1MT320A01 | TGACAGTTGGGCCCTTGTAAG | GCCGGGGATAGGAATTCAAT |
| OMM 3044 | E2 | TCTCTCCCTTGTTCCCCTGA | TCCCCACAGCATAGCATGAG |
| OMM3054 | 1MT288H17 | TGAGCAAGAGAACGAGAGCG | CCTCAGGACCATCAACGACA |
a
Clones numbered with letters only were random BACs isolated from the OSU BAC library. 1MT320A01 is from a clone containing Myd118-1, and 1MT288H17 is from a clone containing ID1B from the Swanson BAC library.