TABLE 2.
Initial, second-round, and marker primers
| Gene | Initial and second-round primers | Marker primers |
|---|---|---|
| Amh | F: AACTGAGTGCGTTCCAGGAG | MF: AGGTGACATGTGCAAGAACAG |
| R: AGTTTTCTTTGCGCGTCGTA | MR: AAGCTGCAGCGGGATACTT | |
| SRF: AGGTGACATGTGCAAGAACAG | ||
| SRR: TCTGCCGACTTCAGAACTTTT | ||
| Dax1 | F: CCGAAACAATAACAGCAGCA | MF: ACGTTGGATGGAAGTAGTTAGGTAAAGTGGC |
| R: GCGACTGGATGTAGTGCAGA | MR: ACGTTGGATGATCCTAAACTTCCACCCCTG | |
| EP: GTAAAGTGGCATGTCTGTAC | ||
| Dmrta2 | F: GAGGTGTTTGGTTCCGTCAG | MF: ACGTTGGATGAAGGCCATCAAAAGCGCATC |
| R: CTCCGTCTTTCAAAGGCTTG | MR: ACGTTGGATGGTCTGTCGCTTTACTGTCTC | |
| EP: GCATCAGCACAGAATCATAAT | ||
| Dmrt2 | F: GCCAAAAGCATCCTCGAA | MF: TGCATCAGAAGATAGACCAGTTT |
| R: GTTTCCAGCTCCTTGTCTGC | MR: CACAAATAAAGTTATTCAAACATGC | |
| SRF: AATTCCAAACACACTCGATGC | ||
| SRR: GAGGGAAACATCGACATCGT | ||
| Fhl3l | F: CGATTGGCTCAAAGTCCTTC | MF: TTGCACACGCTGTAAAAAGG |
| R: TTCTTGGCGTACAGGTTTCC | MR: TCAAAGGTCATGTGGAAATCTG | |
| Foxl2 | F: CGCAATTTGGAGGACAGTTT | MF: ACGTTGGATGGCGCACACACCAAAACAAAC |
| R: CCTTTTTCGGTGCAGCTTAC | MR: ACGTTGGATGTTCACCGATCGAGACAGAAG | |
| EP: CACCAAAACAAACAAGTCC | ||
| Ixl | F: AGAATCGCCTCCCTGAATTT | MF: AGAATCGCCTCCCTGAATTT |
| R: TGTCGATGCTCTGTGAGAGG | MR: CTGAAGTGTCGCTGCTTTTG | |
| Lhx9 | F: GCGGTGGACAAGCAGTGG | MF: ACGTTGGATGTAGCGTTTCAGTTTCACGGG |
| R: TAACGGGCTGACGCTGACTA | MR: ACGTTGGATGACTGGGTCAGTGGATTTAGG | |
| EP: ACGGGCTTGCTCCAAA | ||
| otCYP19 | F: TTTCAGCCGTTCGGTTCAG | MF: TCTCCACATGAGGTTCTTACCC |
| R: TTTCAGTGTTAGCAGGTTTAAATG | MR: TTCACATCATGCATACAGTTTGAG | |
| Sf1 | F: GCGGAAAACCAGGAGTGTAA | MF: GCGGAAAACCAGGAGTGTAA |
| R: AACTTGAAACCGTTGGATCG | MR: CAGGCAAAATGTCCCTGTTT | |
| Sox8 | F: GACTACAAGTACCAGCCTCGG | MF: GGACTGGGAGGAATGAGTGA |
| R: CTGAGCTCGGAGATGTCCAC | MR: CCTGCTGCTGTCAACAAGTC | |
| SRF: CTGGAGCAGAACTGGCTCAT | ||
| SRR: CCTGCTGCTGTCAACAAGTC |
F and R, initial forward and reverse primers; SRF and SRR, second-round forward and reverse primers; MF and MR, marker forward and reverse primers; EP, extension primers for SNP markers.