Figure 4.—

Pole cell number is reduced in dfmr1³ embryos. (A) Wild-type and (B–E) dfmr1³ embryos, 0–4 hr old, were fixed and stained with either Vasa antibody alone (A–C) or with Vasa and Anillin antibodies (D and E). As described in the text, the number of pole cells in dfmr1³ embryos is quite variable. In some dfmr1³ embryos (B), the number of pole cells is equivalent to that seen in wild type, while in other embryos (C), pole cell number is greatly reduced. Some dfmr1³ embryos completely lack pole cells. (D and E) An example of such an embryo: D is the anterior of the embryo, and E is the posterior.