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. 2002 Dec;130(4):1974–1982. doi: 10.1104/pp.010587

Figure 2.

Figure 2

Short-term effects of IAA on GA gene expression. Shown are PsGA3ox1 (A), PsGA2ox1 (B), PsGA2ox2 (C), and PsGA20ox1 (D) transcript levels in the uppermost elongating internode of decapitated peas treated with IAA/lanolin (+) or lanolin only (−). Internodes were harvested 0, 2, 4, 6, and 8 h after application. All plants (except intact controls) were decapitated for 36 h before the commencement of the time course study. IAA/lanolin or lanolin was reapplied 4 h after the initial application. Intact peas (Int) were harvested at the same time as 8-h samples. Corresponding gels showing ribosomal RNAs stained with ethidium bromide are shown immediately below each northern blot, to indicate the loading of lanes. Exposure times were as follows: PsGA3ox1, 48 h; PsGA2ox1, 72 h; PsGA2ox2, 24 h; and PsGA20ox1, 48 h.