TABLE 3.
Crossovers observed in 91 IBM IRILs
No. of crossoversa
|
||||||||
---|---|---|---|---|---|---|---|---|
Group I
|
Group IIb
|
No. of crossovers/μmc | ||||||
Chromosome | BBxMM | MMxBB | Subtotal | BxMxB | MxBM | Subtotal | Total | |
1 | 375 | 383 | 758 | 38 | 29 | 67 | 825 | 17 |
2 | 247 | 260 | 507 | 37 | 28 | 65 | 572 | 14 |
3 | 272 | 284 | 556 | 25 | 31 | 56 | 612 | 16 |
4 | 251 | 245 | 496 | 28 | 25 | 53 | 549 | 15 |
5 | 244 | 265 | 509 | 29 | 21 | 50 | 559 | 16 |
6 | 168 | 161 | 329 | 18 | 16 | 34 | 363 | 12 |
7 | 214 | 223 | 437 | 30 | 20 | 50 | 487 | 17 |
8 | 222 | 208 | 430 | 14 | 20 | 34 | 464 | 16 |
9 | 208 | 184 | 392 | 9 | 18 | 27 | 419 | 16 |
10 | 162 | 155 | 317 | 21 | 22 | 43 | 360 | 16 |
Total | 2363 | 2368 | 4731 | 249 | 230 | 479 | 5210 |
“x” indicates crossover position. B and M stand for B73 and Mo17 alleles, respectively. Crossovers are classified into group I (a single crossover between two pairs of markers from the same parent, i.e., BBxMM or MMxBB) and II (two crossovers flanking a marker, i.e., BxMxB or MxBxM). Within each group, the ratios of the two types (i.e., BBxMM vs. MMxBB or BxMxB vs. MxBxM) are not significantly different from the expected 1:1. The special cases of BxMM and MxBB that can occur at the ends of chromosomes were classified as group I events.
Because there are opportunities for crossovers in multiple generations during the development of IRILs, group II events need not have arisen via double crossovers.
Number of crossovers chromosome per length of pachytene chromosome. Pachytene chromosome lengths are from the inbred KYS (Anderson et al. 2003).