TABLE 2.

p[GT1] insertion lines with aberrant olfactory avoidance behavior

				Mutational effects				P-values from ANOVA
MASa		a/σpb		Sexes pooledc			Sexes separate
Line	Candidate gene	Cytological location	p[GT1] insertion site	♀	♂	♀	♂	S	L	L × S	L♀	L♂
BG01883 (A)	ftz transcription factor 1	75D8-E1	In intron 1	3.6	3.71	1.01	0.72	NS	****	NS	***	NS
BG01918 (A)	Phosphoglycerate kinase	23A3	1.8 kb upstream of exon1	2.89	3.45	1.75	1.02	NS	****	NS	**	*
BG00386 (B)	NMDA receptor 1	83A6-7	302 bp downstream of 3′ region	4.15	3.82	0.21	0.34	NS	*	NS	NS	*
BG01037 (B)	βν integrin	39A1	133 bp in exon 1	3.93	3.92	0.44	0.27	NS	**	NS	*	NS
BG01047 (B)	frizzled	70D4-5	100 bp in exon 1	3.84	4.53	0.53	−0.17	NS	NS	**	**	NS
BG01214 (B)	sugarless/CG10064	65D4-5	275 bp upstream of exon 1	3.91	3.86	0.45	0.30	NS	***	NS	**	*
BG01244 (B)	couch potato	90D1-E1	In intron 2	3.88	4.06	0.49	0.17	NS	*	NS	**	NS
BG01245 (B)	Sema-5c	68F2	197 bp upstream of exon 1	2.97	3.41	1.42	0.63	NS	*****	NS	***	***
BG01279 (B)	CG17836	91D4-5	In intron 3	4.16	3.68	0.20	0.44	NS	**	NS	NS	**
BG01295 (B)	SRY interacting protein 1	54B6-7	17 bp in exon 1	3.48	4.13	0.90	0.12	NS	***	*	***	NS
BG01416 (B)	bicoid-interacting protein 3	42A13-14	In intron 1	4.36	3.65	−0.00	0.47	NS	*	*	NS	**
BG01533 (B)	No predicted gene	67E7		4.32	3.56	0.04	0.53	NS	**	*	NS	**
BG01543 (B)	Merlin	18E1	In exon 1	3.22	3.19	1.17	0.79	NS	*****	NS	***	***
BG01563 (B)	CG16708	82F11-83A1	109 bp upstream of exon 1	3.71	3.88	0.66	0.30	NS	*****	NS	***	***
BG01564 (B)	CG14430	6E4	0.5 kb upstream of exon 1	2.97	3.14	1.42	0.83	NS	*****	NS	***	***
BG01596 (B)	CG13377	1A1	317 bp upstream of exon 1	4.24	3.70	0.12	0.42	NS	*	NS	NS	**
BG01672 (B)	CG14591/SCAP	42A8	27 bp in exon 5	3.19	3.67	1.20	0.45	NS	*****	NS	***	*
BG01686 (B)	1.28	42B3	265 bp upstream of exon 1	3.48	3.60	0.90	0.49	NS	*****	NS	***	**
BG01693 (B)	CG10777/CG10778	7C3-4	25 bp in exon1/1.9 kb downstream of 3′ region	3.48	3.85	0.90	0.32	NS	****	NS	***	*
BG01803 (B)	1.28	42B3	265 bp upstream of exon 1	3.89	3.43	0.48	0.62	NS	****	NS	**	**
BG01909 (B)	CG14035	25C6	3.7 kb downstream of 3′ region	3.65	3.13	0.72	0.83	NS	*****	NS	***	***
BG02022 (B)	CG6301	53D11	1.4 kb downstream of 3′ region	3.22	2.71	1.17	1.14	NS	*****	NS	***	***
BG02053 (B)	Sema-5c	68F2	55 bp upstream of exon 1	1.07	1.66	3.01	1.73	NS	*****	NS	***	***
BG02077 (B)	Rtnl1	25B9-C1	In intron 1	3.68	2.76	0.69	1.10	NS	*****	**	***	***
BG02081 (B)	Rtnl1	25B9-C1	In intron 1	3.23	2.78	1.16	1.09	NS	*****	NS	***	***
BG02115 (B)	Calreticulin	85E	87 bp in exon1	3.76	3.49	0.60	0.58	NS	*****	NS	**	**
BG02169 (B)	High mobility group protein D	57F10	633 bp of transcription initiation site	4.14	3.78	0.22	0.37	NS	*	NS	NS	*
BG02181 (B)	Glutamate dehydrogenase	95C13-D1	126 bp 5′ of transcription initiation site	3.93	3.85	0.43	0.32	NS	**	NS	*	*
BG02200 (B)	CG6782/CG6783	86E10	In intron 1	3.38	3.27	1.00	0.73	NS	*****	NS	***	***
BG02206 (B)	lilliputian	23C1-C3	In intron 2	3.40	4.32	0.98	−0.01	NS	**	***	***	NS
BG02209 (B)	escargot	35D2	321 bp upstream of exon 1	3.55	3.15	0.83	0.82	NS	*****	NS	***	***
BG02251 (B)	innexin 2	6E4	In intron 1	3.23	3.06	1.16	0.88	NS	*****	NS	***	***
BG02314 (B)	1.28	42B3	289 bp upstream of exon 1	3.82	3.61	0.55	0.49	NS	****	NS	**	**
BG02320 (B)	Toll	97D2	44 bp upstream of exon 1	3.78	3.51	0.59	0.57	NS	****	NS	**	***
BG02326 (B)	CG13889	61D2	68 bp in exon 1	4.22	3.73	0.14	0.41	NS	*	NS	NS	*
BG02327 (B)	pipsqueak	47A13-B1	In intron 2	3.29	3.39	1.09	0.65	NS	*****	NS	***	***
BG02348 (B)	Heat shock protein 23	67B3	298 bp upstream of exon 1	3.19	3.15	1.20	0.82	NS	*****	NS	***	***
BG02354 (B)	escargot	35D2	350 bp upstream of exon 1	3.43	3.11	0.95	0.85	NS	*****	NS	***	***
BG02386 (B)	Sema-5c	68F2	81 bp upstream of exon 1	3.55	2.81	0.83	1.06	NS	*****	*	***	***
BG02391 (B)	neuralized	85C2-3	In intron 1	3.97	3.78	0.39	0.37	NS	****	NS	**	**
BG02418 (B)	CG14509	98F13-99A1	In intron 1, 6.8 kb from 5′ region	3.99	3.93	0.38	0.26	NS	*	NS	*	NS
BG02439 (B)	CG32556	16B12-C1	324 bp upstream of exon 1	3.29	2.93	1.09	0.98	NS	*****	NS	***	***
BG02470 (B)	CG8963	53 E4	80 bp in exon 1	3.37	3.20	1.01	0.79	NS	*****	NS	***	***
BG02491 (B)	Ras-related protein	3E5-6	4 kb upstream of exon 1	3.78	4.03	0.59	0.19	NS	**	NS	**	NS
BG02510 (B)	chameau	27F3-4	2.9 kb upstream of exon 1	3.97	3.76	0.39	0.39	NS	**	NS	*	*
BG02522 (B)	CG15816	16C1-C8	876 bp upstream of of exon 1	3.98	3.78	0.39	0.37	NS	**	NS	*	*
BG02566 (B)	Calreticulin	85E1	114 bp in exon 1	3.69	3.74	0.69	0.40	NS	*****	NS	***	***
BG02601 (B)	CG14782/CG14781	2B1	In intron 2/exon 1	3.85	3.54	0.51	0.54	NS	*****	NS	***	***
BG02745 (B)	CG14411/CG14408	12F5	In exon 1	3.96	3.88	0.40	0.30	NS	***	NS	**	*
BG01015 (F)	Moesin	8B4-6	In intron 1	3.97	4.00	0.41	0.33	NS	***	NS	*	*
BG01707 (F)	CG6301	53D11	1.4 kb downstream of 3′ region	3.76	4.39	0.73	0.09	*	**	*	***	NS
BG01084 (F)	CG9238	70E1-2	49 bp upstream of exon 1	4.06	3.66	0.37	0.90	NS	****	NS	*	**
BG01095 (F)	pointed/DNApolymarase E	94E10-13	742 bp in exon 1	4.03	4.08	0.41	0.44	NS	**	NS	*	*
BG01574 (F)	Sema-5c	68F2	82 bp upstream of exon 1	3.93	4.11	0.53	0.40	NS	**	NS	*	*
BG01796 (F)	Spinophilin	62E4-5	In intron 1	3.68	4.35	0.84	0.13	*	**	*	***	NS

Candidate genes used for analysis of epistasis are underlined. Letters in parentheses after the line name denote different co-isogenic Canton S host strains for the p[GT1]-element insertion. *P < 0.05, **P < 0.01, ***P < 0.001, ****P < 0.0001, *****P < 0.00001. P-values < 0.0001 exceed Bonferroni correction for multiple tests.

^aMAS, mean avoidance scores.

^bStandardized mutational effect (see text for explanation).

^cS and L denote the main cross-classified effects of sex and line, respectively, in the ANOVA of avoidance scores.