TABLE 2.
Intragenic gene conversion frequencies for two-factor rec10-155 and rec10-144 homozygous crosses
| rec10 allele | Plasmid | Locus | Mean recombination frequencya | Fold reductionb |
|---|---|---|---|---|
| rec10+ | — | ade6c | 297 ± 114 | — |
| rec10-155 | — | ade6 | 1.2 ± 0.3 | 247 |
| rec10+ | — | lys7d | 14 ± 10 | — |
| rec10-144 | — | lys7 | 0.3 ± 0.2 | 47 |
| rec10-155 | — | lys7 | 0.4 ± 0.4 | 35 |
| rec10+ | — | ura1e | 212 ± 51 | — |
| rec10-155 | — | ura1 | 35 ± 5 | 6 |
| rec10-175 | — | ura1 | 3.0 ± 0.5 | 70f |
| rec10-175 | pSP1g | ura1 | 1.8 ± 1.2 | 118 |
| rec10-175 | pYL167g | ura1 | 24 ± 10 | 9 |
a
Number of prototrophs per 106 viable spores ± 95% confidence interval; n ≥ 4 in all cases.
b
Student's t-test P-values from pairwise comparisons of rec10+ vs. rec10− gave values of P ≤ 0.01 in all cases.
c
Alleles used: ade6-M375 × ade6-52.
d
Alleles used: lys7-1 × lys7-2.
e
Alleles used: ura1-61 × ura1-171.
f
This was the lowest fold reduction obtained in this study for ura1 intragenic recombination. Other mean data values resulted in fold reductions as high as 155-fold.
g
pYL167 is the plasmid carrying the rec10-155 allele (Lin and Smith 1995). pSP1 is a vector control containing no rec10 cloned DNA.