TABLE 5.

Characteristics of the well-studied mutant alleles of rec10

rec10 allele	Mutation	DSB proficient?	Crossover proficiency	Gene conversion proficiency	ade6–M26 hot spot proficiency	LinE status	Other
rec10-175a	Full open reading frame deletion	No	Noneb	Noneb	Noneb	Nonec	—
rec10-109d	Two point mutationsa G526A (V176I) and G533A (G178D)e	Levels of DSBs diminishedf	Regionalgh	Regionalgh	Partialg	Abnormal LinEs formedci	Complements rec10-144jk. Slightly temperature sensitive for recombination at some regionsa
rec10-144jk	Single point mutationj G2180A (G727E)e	Not determined	Limited reduction at all intervals testedl	Limited reduction at all intervals testedjl	Reduced hot spot activityj	Limited abnormal LinEs formedjm	Complements rec10-109jk
rec10-155n	Insertion inactivation mutant; presumed C-terminal truncation of last 103 amino acidsn	Not determined	Limited reduction at all intervals testedl	Limited reduction at all intervals testedln	Reduced hot spot activityo	Noneimp	—

Other rec10 mutant alleles have been isolated/generated and sequenced (De Veaux et al. 1992; Ellermeier and Smith 2005; our unpublished data), but they have undergone only limited analysis to date and are not listed here.

^aEllermeier and Smith (2005).

^bNone equates to the levels observed for a rec12 null mutant.

^cAs measured by immunocytochemistry.

^dPonticelli and Smith (1989).

^eNumbered from the start of the open reading frame.

^fCervantes et al. (2000).

^gDe Veaux and Smith (1994).

^hKrawchuk et al. (1999).

ⁱLorenz et al. (2004).

^jPryce et al. (2005).

^kDe Veaux et al. (1992).

^lThis study.

^mAs determined by immunocytochemistry and electron microscopy.

ⁿLin and Smith (1995).

^oD. W. Pryce and R. J. Mc Farlane (unpublished observation).

^pMolnar et al. (2003).