Figure 3.

Fe³⁺- and Cu²⁺-chelate reductase activities of C. reinhardtii, grown in Fe- and Cu-sufficient and deficient media. The cells were grown for 1 d without Fe and up to 6 d without Cu. During the Cu-deficient growth, Tris-acetate-phosphate (TAP) medium was renewed every 2nd d and the cells were diluted to an OD₇₅₀ of 0.2. Fe³⁺-chelate (A) and Cu²⁺-chelate (B) reductase activities were determined. Values represent the mean of three independent assays. ses are shown.