Figure 3.

Expression of ATS1, APR1, SAT1, and CYS1 in wild-type and sac mutants. Cells were grown to mid-log phase in sulfate-replete medium, washed twice with sulfur-deficient medium, and were then incubated for 0, 1, 2, 4, 8, or 12 h prior to harvesting RNA. One microgram of RNA from the indicated samples was separated by electrophoresis, blotted onto a membrane, and hybridized separately with the indicated cDNA clones. Hybridization with a genomic clone of RBCS1 was also performed as a control.