Skip to main content
. 2002 Dec;130(4):2076–2084. doi: 10.1104/pp.012484

Figure 7.

Figure 7

Expression of ARS1 and ARS2 in wild-type and sac mutants. Wild-type (A), sac1 (B), sac2 (C), and sac3 (D) were grown to mid-log phase in sulfate-replete medium, washed twice with sulfur-deficient medium, and were then incubated for 0, 1, 2, 4, 8, or 12 h prior to harvesting RNA. One microgram of RNA was separated by electrophoresis, blotted onto a membrane, and hybridized separately with cDNA clones of ARS1 and ARS2. Hybridization with a genomic clone of RbcS1 was also performed as a control. E, Overexposure of the blots shown in D shows that ARS1 and ARS2 are expressed in sac3 cells grown in sulfur-replete medium.