Alignment of the deduced amino acid
sequences for the SoGA3ox1 gene from spinach with other GA
3-oxidases. Conserved regions are boxed in black. Asterisks indicate
the putative Fe2+-binding motif at the active
site of 2-oxoglutarate-dependent dioxygenases (Thomas et al.,
1999). SoGA3ox1, GA 3-oxidase from spinach (GenBank accession
no. AF506280); NtGA3ox, GA 3-oxidase from tobacco
(Nicotiana tabacum; accession no. BAA89316); StGA3ox, GA
3-oxidase from potato (Solanum tuberosum;
accession no. AAK91507); PsGA3ox, GA 3-oxidase from garden pea (GenBank
accession no. AAC96015); AtGA3ox, GA 3-oxidase from Arabidopsis
(accession no. T51691); CmGA3ox and CmGA23ox, GA 3-oxidases from
pumpkin (Cucurbita pepo; accession nos. AAB64347 and
CAB92914); and OsGA3ox, GA 3-oxidase from rice (Oryza
sativa; accession no. BAB62072).