Alignment of the deduced amino acid
sequences for the SoGA2ox1 and SoGA2ox2 genes
from spinach with other GA 2-oxidases. Conserved regions are boxed in
black. Asterisks indicate the putative
Fe2+-binding motif at the active site of
2-oxoglutarate-dependent dioxygenases (Thomas et al.,
1999). SoGA2ox1 and SoGA2ox2, GA 2-oxidases from spinach
(accession nos. AF506281 and AF506282); PcGA2ox1, GA 2-oxidase from
runner bean (accession no. AT132438); PsGA2ox1 and PsGA2ox2, GA
2-oxidases from garden pea (accession nos. AF100954 and AF100955);
AtGA2ox1, AtGA2ox2, and AtGA2ox3, GA 2-oxidases from Arabidopsis
(accession nos. AJ132435, AJ132436, and AJ132437); OsGA2ox1, GA
2-oxidase from rice (accession no. BAB40934).