Figure 4.

Expression patterns of the SoGA20ox1, SoGA3ox1, SoGA2ox1, and SoGA2ox2 genes in various organs of spinach plants grown in SD and after 8 LD. Northern blots were prepared by separating 30 μg of total RNA of each organ through 1.2% (w/v) agarose gels containing formaldehyde, followed by transfer to Hybond-N⁺ membranes. The blots were separately hybridized to ³²P-labeled cDNAs of SoGA20ox1, SoGA3ox1, SoGA2ox2, or SoActin. After RT-PCR of SoGA2ox1 and SoActin, 10 μL of the PCR reaction products were separated through 2.0% (w/v) agarose gel by electrophoresis and hybridized to a ³²P-labeled cDNA of SoGA2ox1 or SoActin. The numbers under the blots indicate the relative amount of each transcript after standardization using SoActin as a loading control. The value for each gene transcript in blades of spinach grown in SD conditions was arbitrarily set at 1.0.