Table II.

Substrate specificity of the purified and recombinant β-primeverosidases

Substrate	Relative Activity
	Tea β-primeverosidase	Recombinant β-primeverosidase
	%
2-Phenylethyl β-primeveroside	100	100
2-Phenylethyl β-glucoside	0	0
p-Nitrophenyl β-primeveroside	48	62
p-Nitrophenyl β-glucoside	0.5	0.3
p-Nitrophenyl β-xyloside	0	0

Activity was determined by measuring the release of 2-phenyl ethanol by HPLC or p-nitrophenol by absorbance at 405 nm. Substrates were used at 10 mm concentrations. Relative activity shows the percentage activity detected using 2-phenylethyl β-primeveroside as a substrate.