Root hairs of the act2-1 mutant Arabidopsis plants. a through d, Complementation of the act2-1 root hair phenotype in plants expressing various actin transgenes. a, Seven-day-old act2-1 mutant seedlings display wild-type filamentous roots when transformed with genomic tACT2g fragment (middle seedling). Flanking seedlings are homozygous act2-1 mutant segregants that did not contain the complementing tACT2g transgene. b, Seven-day-old act2-1 mutant seedlings display wild-type filamentous roots when transformed with genomic tACT7g fragment (middle seedling). Flanking seedlings are homozygous act2-1 mutants. c, Seven-day-old act2-1 mutant seedlings display wild-type filamentous roots when transformed with tACT1c (Fig. 1d), ACT1 cDNA under expression of ACT2 regulatory regions (middle seedling). Flanking seedlings are homozygous act2-1 mutants. d, Root hair length in wild-type, act2-1, and complemented lines. n = 20 for each. ses are indicated. e though j, Immunocytochemistry of wild-type (e and f) and act2-1/act2-1 mutant Arabidopsis root hairs (g–j). Bar in a through c = 1 mm. Bars in e through j = 10 μm.