Figure 4.

Map-based cloning of the DER1 locus. A, In an initial mapping population, the DER1 locus was located on chromosome 3, in an interval between the SSLP markers nga162 and uzu1 at 20 and 34 cM, respectively. B, Using a larger mapping population, DER1 was mapped to a region flanked by the markers uzu23 and uzu30 and spanned by the bacterial artificial chromosome (BAC) clones MVE11 and MCB22. The ACT2 gene is located on the BAC MVE11. C, A model of the actin protein structure in the ADP-bound form. Tetramethylrhodamine-5-maleimide shown in the right lower part was used to facilitate crystallization (Otterbein et al., 2001). The domains that harbor the two amino acids mutated in the der1 alleles are circled.