Figure 1.

A, Physical map of the genomic clone (OsCc1) encoding a rice cytochrome c. The solid boxes represent exons. A partial cDNA (cyt) that was used as a probe for RNA-blot experiments is indicated. The transcriptional start site and the translational start codon are marked with +1 and ATG, respectively. Sequences that are similar to those of CRE and NRF1-binding sites are indicated by I and II, respectively. Important restriction enzyme sites are abbreviated as follows: C, ClaI; RI, EcoRI; S, SalI; and X, XbaI. B, The expression of OsCc1 in different tissues of rice. RNA-blot analysis was carried out using total RNA from rice suspension cells (SC), leaves (L), roots (R), and calli (C). C, The response of OsCc1 mRNA expression to the Bt₂cAMP treatment. Suspension cells (SC), leaves (L), and roots (R) were treated with 1 mm dibutyryl cAMP (Bt₂cAMP) for the time periods indicated, and total RNA was extracted. Total RNA was fractionated on a denaturing agarose gel, blotted to a nylon membrane, and hybridized with a [³²P]-labeled cyt cDNA probe, shown in A (OsCc1). The loading of an equal amount of total RNA in each lane was verified by ethidium bromide staining (EtBr).