Figure 2.

The response of OsCc1 mRNA expression in etiolated seedlings to light exposure. Etiolated seedlings were treated with light at 150 μmol m² s⁻¹ for the time periods indicated, and total RNA was extracted. Total RNA was fractionated on a denaturing agarose gel, blotted to a nylon membrane, and hybridized with either a [³²P]-labeled cyt cDNA probe, shown in Figure 1A (OsCc1), or the coding region for a small subunit of ribulose-1,5-bisphosphate carboxylase (RbcS). The loading of an equal amount of total RNA in each lane was confirmed by ethidium bromide staining (EtBr). Lane L shows total RNA extracted from mature leaves.