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. 2002 Aug;129(4):1473–1481. doi: 10.1104/pp.002261

Figure 3.

Figure 3

The expression vectors used for rice transformation. pSB-CG (OsCc1::sgfp) consists of the OsCc1 promoter linked to the sgfp coding region, the 3′ region of the potato proteinase inhibitor II gene (3′pinII), and the bar gene expression cassette that contains a 35S promoter/bar coding region/3′ region of the nopaline synthase gene (3′nos). pSBG700 (Act1::sgfp) and pSB-RG (RbcS::sgfp) are identical to pSB-CG except that the rice Act1 and RbcS promoters, respectively, are fused to the sgfp-coding region. Important restriction sites are indicated: EcoRV (E), NcoI (N), XbaI (X), BamHI (B), and SpeI (S). Restriction enzymes followed by the expected fragments and hybridization probe (probe) used for genomic DNA-blot analyses are shown below the map.