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. 2002 Aug;129(4):1473–1481. doi: 10.1104/pp.002261

Figure 4.

Figure 4

Genomic DNA-blot analysis of transgenic rice plants. A, Genomic DNAs from the leaf tissues of three (1–3) independent lines of OsCc1::sgfp-transformed plants were digested with NcoI (N), EcoRV (E), and hybridized with a 0.7-kb DNA fragment containing the sgfp coding region (see Fig. 3). PC contains NcoI-digested pSB-CG. B, Genomic DNAs from the leaf tissues of three (1–3) independent lines of Act1::sgfp-transformed plants were digested with BamHI (B), XbaI (X), and hybridized with the same probe described in A. PC contains BamHI-digested pSBG700. C, Genomic DNAs from the leaf tissues of three (1–3) independent lines of RbcS::sgfp-transformed plants were digested with XbaI (X), SpeI (S), and hybridized with the same probe described in A. PC contains XbaI-digested pSB-RG. NC, Genomic DNAs from an untransformed control plant; 1X, 3X, and 5X in PC represent one, three, and five genome equivalents of pSB-CG (A), pSBG700 (B), or pSB-RG (C), relative to 5 μg of rice genomic DNA, respectively. The DNA molecular size markers (M) are indicated.