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. 2002 Aug;129(4):1473–1481. doi: 10.1104/pp.002261

Figure 6.

Figure 6

RNA gel-blot analysis and relative levels of sgfp transcripts in transgenic rice plants shown in Figure 4. A, Total RNAs extracted from leaf, root and callus of three (1–3) independent lines of OsCc1::sgfp-, Act1::sgfp-, and RbcS::sgfp-transformed plants and from an untransformed control plant (NT) were hybridized with a 0.7-kb DNA fragment containing the sgfp coding region (see Fig. 3). Hybridizations with the rice RL5 encoding the 5S rRNA-binding protein (Kim and Wu, 1993) were used for equal RNA loading. B, Transcript levels of sgfp shown in A were calculated using those of corresponding RL5 as a reference and the resultant values were then normalized to 1 for that from leaf tissues of Act1::sgfp-transgenic line 1.