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Complementation of a yeast TRK1/TRK2 mutant with McHAK1, McHAK2, and McHAK4. The yeast strain CY162 (Ko and Gaber, 1991) was transformed with either the empty vector pYES2 or with McHAK1, McHAK2, and McHAK4, respectively, cloned into the yeast expression vector pYES2. Positive transformants were grown on Gal-SC containing 3 mm K+ (A) or 7 mm K+ (B), or Gal-SC supplemented with 150 mm RbCl, 150 mm NaCl, or 15 mm CsCl.
