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The effect of Arf1[T31N] on the localization of constitutively expressed ST:GFP. Protoplasts obtained from transgenic plants constitutively expressing ST:GFP were transformed with the constructs indicated. The effect of these Arf1 proteins on the localization of ST:GFP was examined 24 h after transformation. Green and red images are GFP and RFP signals, respectively. The blue signals in a, b, and c indicate autofluorescence of chlorophyll. Bar = 20 μm.
