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The effect of BFA on the targeting of H+-ATPase:GFP. Targeting of H+-ATPase:GFP was examined in protoplasts in the presence (+BFA) and absence (−BFA) of 30 μg mL−1 BFA. BFA was added right after transformation. Green and red images are GFP and chlorophyll, respectively. N and CH indicate nucleus and chloroplast, respectively. Arrows indicate H+-ATPase:GFP.
