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Induction of HFi22 mRNA accumulation upon infection of plants with TBSV. Total RNA was collected from healthy (H) N. benthamiana and N. tabacum or from plants 9 d after inoculation with TBSV (T). RNA was electrophoresed, and loading was verified based on an EtBr-staining signal obtained for the 28S ribosomal RNA (rRNA). RNA was transferred to a membrane and hybridized to an HFi22 cDNA specific probe.
