Table I.

Interaction between HFi22 and various proteins upon cotransformation of yeast with corresponding plasmids

HFi22 Plusa	–b	P22	P19	P33	CP	22p	P6.6	Lamin
Colony growth in L−, T− medium	+++	+++	+++	+++	+++	+++	+++	+++
Interactionc	–	+++	+	–	–	–	–	–

^a The HFi22-expressing plasmid (p3.5) was cotransformed with bait plasmids expressing either TBSV P22, P19, P33 or CP, and the 22p plasmid contains p22 in the reverse orientation. Two other control bait plasmids expressed P6.6 of Panicum mosaic virus or lamin. 

^b In this column, only the HFi22-expressing prey plasmid was present, and these colonies were prototrophic only for leucine, whereas for the other columns colonies were also prototrophic for tryptophan because both prey and bait plasmids were present. 

^c The results represent the combined data of growth on histidine-depleted medium and the β-gal assay on colonies grown in parallel in presence of histidine. The +++ indicates rapid growth in absence of histidine and that a blue color appeared 1.5 h after the start of the β-gal reaction. A + indicates a slower growth rate under histidine selection and that the blue color appeared after prolonged incubation (>10 h). A minus (–) indicates no growth in absence of histidine and no blue color developed upon an extended β-gal reaction.