Figure 2.

Southern blot of double-resistant plants revealing stable inserts with a BAR gene probe. A, Double-resistant progeny pools from different first transformants (WATs) are displayed. The bands show independent insertions. A ladder showing the size in kilobase pairs is indicated on the left side. The numbers of plants per pool are: WAT 8, seven plants; WAT 10, eight plants; WAT 14, six plants; WAT 15, eight plants; WAT 18, 10 plants; WAT 20, eight plants; and WAT 21, nine plants. B, Southern blot of random individual plants derived form different first transformants (population 1). C, Plants selected from spraying F₂ (obtained by outcrossing T₃ plants) seeds of line WAT8, obtained in population 5.