Figure 2.

RNA-blot hybridization analysis of CER6 expression in developing Arabidopsis stems and leaves. In each case, 10 μg lane⁻¹ of total RNA was probed with CER6 coding region and 18S rRNA (loading control). A, RNA extracted from 8-d-old seedlings (S) and from 2-cm segments of the tops (T) and bottoms (B) of bolting stems 4, 10, and 25 cm (when flower production had ceased) tall. RNA was also extracted from the 2-cm section at the center (M) of 25-cm-tall stems. B, RNA extracted from cotyledons (C), rosette (in sequence; first initiated, 1; last initiated, 10), and cauline leaves (also in sequence; numbering as with rosette leaves). Leaves were harvested when the plants had bolting stems 10 cm tall.