Figure 7.

Light is required for CER6 expression in seedlings and in bolting stems, as shown by RNA-blot hybridization. Ten micrograms of total RNA was loaded into each lane. A, RNA extracted from wild-type (Columbia-2), det1, and det2 mutant 8-d-old seedlings germinated on agar plates in continuous light (L), continuous darkness (D), and dark for 6 d followed by transfer to light for the last 2 d (6D + 2L; wild type only). The blot was probed with the CER6 coding region and 18S rRNA (loading control). B, RNA extracted from stem tops of plants incubated in continuous light (L) or placed in darkness for 8, 24, 48, or 96 h. The blot was hybridized to the CER6 5′-UTR and 18S rRNA probes.