Figure 8.

Water deficit and ABA treatment increase CER6 transcript accumulation in seedlings and in bolting stems of Arabidopsis. RNA-blot hybridization of total RNA (10 μg lane⁻¹) probed with CER6 5′-UTR, rd29A unique sequence, and 18S rRNA (loading control). A, RNA extracted from the tops of 10 cm tall bolting stems of plants grown in well-watered (W) pots and in pots allowed to dry out immediately after transplanting (D). B, RNA extracted from shoots of 14-d-old seedlings germinated on agar plates, then transferred to and incubated for 10 h in liquid AT medium containing PEG (0%–20%) prior to harvest. C, RNA extracted from shoots of 14-d-old seedlings germinated on agar plates, then transferred to and incubated for 10 h in liquid AT medium containing NaCl (0–200 mm) prior to harvest. D, RNA extracted from shoots of 14-d-old seedlings germinated on agar plates, then transferred to and incubated for 10 h in liquid AT medium containing ABA in methanol (10⁻⁴ m) or an equal volume of methanol (control). E, RNA extracted from the tops of 10 cm tall bolting stems of wild-type (Columbia-2 and Landsberg erecta) and ABA synthesis mutants (aba1-1, aba1-3, aba1-4, aba2-2, and aba3-2).