Figure 2.

RNA gel-blot analysis of transcripts of defense-related genes in wild-type and AATP1(St) antisense tuber tissue. A, Total RNA was extracted from discs of stored (4 month, 8°C) wild-type or AATP1(St) antisense tubers (line JT 654) directly after punching (0 h). As an alternative, discs were conditioned for 4 h in sterilized water (4, water control) for 1 more (+1, PAL 23 mRNA analysis) or for 9 more h (+9, CHI B3 and A2, BGL B2, and A1 mRNA analysis) in water, Pmg elicitor, or E. carotovora supernatant. Pmg elicitor was used at 40 μg mL⁻¹. The supernatant of an E. carotovora culture (Eca supernatant) was used as described in “Materials and Methods.” B, Total RNA was isolated from discs of the stored wild-type or AATP1(St) antisense tubers directly after punching (0 h). As an alternative, discs were conditioned for 4 h in sterile water (4, water control) and then treated with 40 μg mL⁻¹ Pmg elicitor. At various time points postelicitation, total RNA was extracted from an aliquot of discs and analyzed for the accumulation of BGL B2 transcripts by RNA gel-blot hybridization.