Table I.
The effect of daylength and temperature to ABA content of the buds of the WT, the line 8, and the line 22 hybrid aspen overexpressing oat PHYA
| Line | Daylength | 7 Weeks at 18°C | 10 Weeks at 18°C | 5 Weeks at 18°C and 2 Weeks at 0.5°C | 5 Weeks at 18°C and 5 Weeks at 0.5°C |
|---|---|---|---|---|---|
| μg g−1 dry wt | |||||
| WT | LD | 14.08 | 18.29 | 26.76 | 6.18 |
| Line 8 | LD | 24.29 | 18.59 | 20.94 | 4.0 |
| Line 22 | LD | 5.15 | 9.37 | 11.54 | 2.48 |
| WT | SD | 4.18 | 1.48 | 11.58 | 2.53 |
| Line 8 | SD | 4.97 | 1.57 | 11.35 | 2.95 |
| Line 22 | SD | 4.48 | 7.3 | 10.58 | 0.54 |
Plants were grown for 5 weeks at 18°C under LD (16 h) or SD (10 h) conditions, after which they were left to the same temperature or transferred to 0.5°C. Samples were collected after 2 and 5 weeks of transfer. Values are means of two replicate samples, each consisting of five to 18 buds. ANOVA: Line (L), P = 0.0001; Daylength (D), P ≤ 0.0001; Temperature (T), P ≤ 0.0001; L × D, P ≤ 0.0001; L × T, P = 0.038; D × T, P = 0.025; L × D × T, P = 0.025.