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Fluorescent probes report the presence of NO in barley aleurone protoplasts. Epifluorescence (A–F) and differential interference contrast (G–I) images of barley aleurone protoplasts incubated with DAQ (A), 1-AQ (B), DAF-2 DA (D and G), 1-AF DA (E and H), or no probe (C, F, and I). Arrows point to damaged cells.
