Figure 3.

Changes in c-src PTK activity and Tyr phosphorylation after training. c-src PTK activities were measured in both synaptosomal (A) and synaptic membrane (B) fractions (n = 3; **, P < 0.001). Changes in Tyr phosphorylation were measured in synaptosomal (C) and synaptic membrane (D) fractions by immunoprecipitation by using an anti-phosphotyrosine antibody. The intensity of the protein band correlated with in vivo Tyr phosphorylation of pp60c-src (P-pp60c-src) (n = 3; *, P < 0.001). See Fig. 2 for details on S1, S24, T1, and T24.