Figure 5.

Binding of pp60c-src to IR after training. (A) Synaptic membrane proteins were precipitated with anti-IR antibody, and the coprecipitated pp60c-src was identified on Western blots with anti-c-src antibody (n = 3; **, P < 0.001). (B) Effects of Ca²⁺ and phorbol ester on protein–protein interaction of pp60c-src. Hippocampal synaptosomal proteins were phosphorylated in vitro under conditions optimal for Ser/Thr (lanes 1–4) or Tyr (lanes 5–7) phosphorylations in the presence or absence of Ca²⁺ and phorbol 12-myristate 13-acetate. The phosphorylated proteins were immunoprecipitated by anti-c-src antibody followed by identification on Western blots with anti-NR2 (Top) and synapsin I (Middle), respectively. Samples also were precipitated with anti-synapsin I antibody and subsequently detected with c-src antibody (Bottom).