Southern hybridization analysis of nine representative T1 transgenic lines harboring silencing transgenes. From left: lane 1, Δ9-HP*6; lane 2, Δ9-HP*51; lane 3, Δ9-HP*62; lane 4, Δ9-HP*150; lane 5, Δ9-AS*118; lane 6, Δ12-HP*23; lane 7, Δ12-HP*83; lane 8, Δ12-HP*128; and lane 9, Δ12-HP*124. Genomic DNA from each line was digested with HindIII and probed with the whole promoter fragment of the soybean lectin gene. The bands represent the transgene insertions. The migration of DNA size markers is shown on the left in kb.