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. 2002 Aug;129(4):1852–1857. doi: 10.1104/pp.010963

Figure 2.

Figure 2

Functional complementation of the COX17 yeast mutant. Yeast strains are as follows: 1, wild-type W303; 2, respiratory-deficient mutant W303ΔCOX17; 3, respiratory-deficient mutant W303ΔCOX17 transformed with a wild-type COX17 gene from yeast; 4, empty pYPGE15 yeast expression vector; 5, respiratory-deficient mutant (W303ΔCOX17) transformed with the AtCOX17 cDNA cloned in sense orientation into pYPGE15; and 6, respiratory-deficient mutant (W303ΔCOX17) transformed with the AtCOX17 cDNA cloned in antisense orientation into pYPGE15. Yeast strains were grown in yeast peptone dextrose Glc medium (left plate) or in yeast extract peptone dextrose glycerol containing medium (right plate). Plates were photographed at 3 d after being grown at 30°C.