Figure 1.

The level of leaf mitochondrial AOX protein in wild-type and transgenic (AS8 and S24) plants. AS8 plants contain an antisense AOX transgene that results in a silencing of leaf AOX expression, whereas S24 plants contain a sense AOX transgene that results in constitutive high levels of leaf AOX protein. Plants were either left untreated (−) or were treated for 12 h with 2 mm SA in their hydroponic medium prior to mitochondrial isolation. Mitochondrial protein (100 μg) was separated by SDS-PAGE, transferred to nitrocellulose, and probed with a monoclonal antibody against AOX. Typical results are shown.