Figure 3.

The level of leaf mitochondrial AOX protein in wild-type (A), AS8 (B), and S24 (C) plants grown in hydroponic culture. The (+SA) plants were grown with 0.1 mm SA in their hydroponic medium for 7 d prior to TMV inoculation and throughout the postinoculation period. Numbers refer to DPI, whereas the M refers to mock-inoculated plants, in which case mitochondria were isolated at 10 DPI. Mitochondria were isolated from leaves 2 and 3 above the inoculated leaf, and mitochondrial protein (40 μg) was separated by SDS-PAGE, transferred to nitrocellulose, and probed with a monoclonal antibody against AOX. Typical results are shown.