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Northern-blot analysis of HAST expression in maize roots. Total RNA was extracted from roots of sulfur-starved (−S), control (C), and Cd-exposed (Cd) plants. Thirty micrograms of total RNA was loaded onto each lane. Blots were hybridized with 32P-labeled HAST probe. Ribosomal RNAs were stained on the gel with ethidium bromide (Et-BR) and used to check loading.
