Table I.
Effects of Cd2+ exposure on the concentrations of NPT, Cys, γEC, GSH, and PCs in maize roots
| Thiol Concentrations
|
|||||
|---|---|---|---|---|---|
| 0 h
|
24 h
|
48 h
|
|||
| Control | Control | Cd | Control | Cd | |
| nmol GSH equivalent g−1 fresh wt | |||||
| NPT | 231.3 ± 10.3 | 222.2 ± 10.1 | 464.5 ± 17.4 | 199.0 ± 7.2 | 1,159.3 ± 50.2 |
| Cys | 9.3 ± 0.3 | 8.9 ± 0.2 | 12.0 ± 0.6 | 8.1 ± 0.5 | 15.6 ± 0.6 |
| γEC | 3.6 ± 0.2 | 4.1 ± 0.3 | 12.6 ± 0.4 | 4.2 ± 0.3 | 28.7 ± 1.2 |
| GSH | 198.4 ± 7.0 | 194.3 ± 4.3 | 112.3 ± 7.9 | 188.2 ± 11.4 | 105.3 ± 6.6 |
| PCs | – | – | 327.6 ± 19.1 | – | 1,009.7 ± 50.6 |
Plants were grown for 24 or 48 h in a complete nutrient solution supplemented or not with 10 μm CdCl2. At different times, roots were excised from shoots, grounded in liquid N2, and an HCl extract was analyzed for thiol content. Values are means ± se of three experiments run in triplicate (n = 9).