Figure 7.

Yeast two-hybrid system demonstrating OsMEK1-OsMAP1 interaction. The entire OsMEK1 open reading frame was fused in-frame to the C terminus of the DNA-binding domain of LexA in pHybLex/Zeo (Invitrogen) and was used as a bait. The open reading frames of OaMAP1, OsMAP2, and OsMAP3 were individually fused in-frame to the C terminus of the transcriptional activation domain of B42 in pYES-Trp2 (Invitrogen) and were used as preys. The resulting bait- and prey-containing plasmids were cotransformed into yeast strain L40 according to the manufacturer's method. c-Fos and c-Jun are two proteins that are know to interact and are used as a positive control. The growth of streaked yeast cells transformed with different plasmid combination was prototrophically assayed on a selective medium lacking Trp, Leu, and His.