Figure 2.

NAE inhibition of recombinant castor bean PLDα expressed in E. coli. Addition of various NAE types to PLD assays inhibited the hydrolytic activity of PLD measured toward PC substrate. Protein (20 μg, E. coli lysate) was added to initiate the reaction. NAE types were included at 0.1 to 1.0 μm, and included NAE 14:0 (●) and NAE 12:0 (▪). Data points are the averages of duplicate samples with the range generally less than 10%. Results plotted are from a single experiment and are representative of replicate experimental results.