Figure 5.

Comparison of NAE effects on recombinant castor bean PLDα, recombinant Arabidopsis PLDβ, and recombinant Arabidopsis PLDγ expressed in E. coli. Assays were conducted as described in “Materials and Methods.” Relative PLD enzyme activity is plotted for comparative purposes in the presence of 10 μm NAE 12:0 or NAE 14:0. Bars represent the mean and sd of three independent experiments. Control activity is the activity toward PC substrate alone; for PLDα, it was the rate of PC hydrolysis (1.3 μmol h⁻¹ mg⁻¹ protein), and for PLDβ/γ, it was rate of PC transphosphatidylation (β, 85 nmol h⁻¹ mg⁻¹ protein; γ, 72 nmol h⁻¹ mg⁻¹ protein).