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Analysis of protease-digested glycoprotein by reversed-phase HPLC. Peptides were obtained after digestion of reduced and alkylated glycoprotein with trypsin or protease V8. A, HPLC profile of trypsin-digested glycoprotein. B, HPLC profile of protease V8-digested glycoprotein. Numbers indicate the peptides that have been sequenced. Triangles indicate peptides blocked at the N terminus.
