Table I.

N-terminal and peptide sequences of the glycoprotein, α - and β -megaspermin

Glycoprotein
N-T	EACSASEQASAYTSMVGLLQGTALSTCASDSGYNMLYA
T1	SLADNFEPGCTSSHyPA*DAHyPVA**AAHyPS
T2	SLADNFEPGCTSSPA*DAPVA**AAPSP
T3	VDACHELIK
T4	YATSLPTAEQTAAMCK
T5	NVQATNPPDCDLNIPTSGAVMNVK
T6	EACSASEQASAYTSMVGLLQGTALST
V8-1	QTAAMCKVDACHE
V8-2	LIKNVQATNPPDCDLNIPTSGAVMNVKSLADNFEPG*TSS*A*DA*VA
V8-3	VGLLQGTALSTCASDSGYNML
V8-4	EACSASEQASAYTSMVGLLQGTALSTCASDSGYNMLYATSLPTA
Ps	EACSASEQASAYTSMVGLLQGTALSTCASDSGYNMLYATSLPTAEQTAAMCKVDACHELIKNVQATNPPDCDLNIPTSGAVM  NVKSLADNFEPGCTSS(P/HyP)A*DA(P/HyP)VA**AA(P/HyP)SP
α-Megaspermin
N-T	TTCTTTQQTAAYVALVSILSDASFNQCATDSGYSMLTATS
T1	LMCASTACNSMIAK
T2	IITLNAPDCELTVPTSGLVLNVYSYA
T3	TTCTTTQQTAAYVALVSILSDASFNQCATDSGYSMLTATSLPTTDQY
Ps	TTCTTTQQTAAYVALYSILSDASFNQCATDSGYSMLTATSLPTTDQYKLMCASTACNSMIAKIITLNAPDCELTVPTSGLVLNVYSYA
β-Megaspermin
N-T	TACTATQQTAAYKTLVSILSDASFNKCSTDSGYSMLTAKALPTNAQYKLMCAST

The glycoprotein was digested with trypsin (T) or V8 protease (V8), and α -megaspermin with trypsin. The nos. refer to the peptides in Figure 1. N-T, N-terminal sequencing of the protein. ^*, Lack of amino acid detection. HyP, Hydroxy-Pro; (P/hyP), Pro or HyP. A partial primary structure (Ps) was deduced from peptide alignment for the glycoprotein and after comparison with capsicein amino acid sequence for α -megapsermin.