Figure 3.

Northern analysis of mud transcripts. Each panel presents a single blot hybridized sequentially with the indicated probes, which are described in Fig. 1. Samples 1 and 2 are RNA isolated, respectively, from male and female third instar larvae; sample 3 is RNA isolated from unstaged embryos. Different embryonic and larval RNA preparations were used in A and B. Note that the smallest larval transcript (detected only by probes A, B, and C) is predominantly expressed in males. Two exposures are shown of the blot hybridized with probe C; the one on the right has been overexposed to illustrate that the small message is expressed in female larvae, albeit at very low levels.