Table I.
Metabolite amounts of French bean leaves maintained in darkness at three temperatures
| Metabolite | Initial Composition | 1 h in Dark
|
6 h in Dark
|
End of Dark Period
|
||||||
|---|---|---|---|---|---|---|---|---|---|---|
| 10°C | 20°C | 30°C | 10°C | 20°C | 30°C | 10°C | 20°C | 30°C | ||
| Citrate | 50 ± 7 | 54 ± 1 | 50 ± 5 | 40 ± 2 | 13 ± 3 | 100 ± 9 | 61 ± 14 | 64 ± 41 | 52 ± 19 | 41 ± 16 |
| Malate | 310 ± 112 | 230 ± 11 | 270 ± 43 | 157 ± 6 | 83 ± 22 | 256 ± 10 | 73 ± 5 | 10 ± 7 | 140 ± 39 | 13 ± 1 |
| Gluconate | 0 | 0 | 3 ± 1 | 0 | 2 ± 1 | 0 | 2 ± 1 | 9 ± 1 | 4 ± 1 | 4 ± 1 |
| Glc | 1,467 ± 565 | 1,198 ± 18 | 1,418 ± 140 | 1,436 ± 22 | 686 ± 234 | 1432 ± 26 | 455 ± 118 | 84 ± 18 | 273 ± 85 | 51 ± 5 |
| Gal | 120 ± 49 | 243 ± 4 | 354 ± 29 | 286 ± 1 | 137 ± 44 | 338 ± 31 | 187 ± 52 | 100 ± 29 | 179 ± 62 | 143 ± 20 |
| Myoinositol | 7 ± 2 | 16 ± 1 | 18 ± 1 | 18 ± 1 | 8 ± 3 | 17 ± 1 | 61 ± 9 | 7 ± 2 | 51 ± 11 | 0 |
| Glycerol | 82 ± 10 | 114 ± 5 | 181 ± 32 | 100 ± 8 | 44 ± 7 | 143 ± 4 | 87 ± 18 | 46 ± 1 | 69 ± 17 | 51 ± 5 |
| Linolenate | 17 ± 3 | 30 ± 1 | 31 ± 1 | 29 ± 1 | 10 ± 5 | 32 ± 1 | 22 ± 2 | 10 ± 1 | 20 ± 2 | 9 ± 1 |
| Palmitate | 5 ± 1 | 10 ± 1 | 11 ± 1 | 10 ± 1 | 4 ± 1 | 11 ± 1 | 10 ± 2 | 5 ± 1 | 8 ± 2 | 6 ± 1 |
Metabolites were simultaneously identified and quantified with the GC-MS procedure. Their amounts are given relative to an internal standard added in each sample. Glc signal integrates free Glc and also Glc from starch and Suc. Data are means of three independent measurements ± se.