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. 2003 Jan;131(1):287–297. doi: 10.1104/pp.013029

Figure 2.

Figure 2

Sequence comparison of BR6ox2 and related genes. A, Sequence alignment of the BR6ox2, BR6ox1, and tomato Dwarf genes. Reverse contrast characters highlight identical amino acid residues and hatched characters indicate conserved residues. The GenBank/EMBL/DDBJ accession numbers of the BR6ox1 (CYP85A1) and the tomato Dwarf gene (CYP85A1) are AB035868 and U54770, respectively. The heme-binding signature sequence is underlined. B, Phylogenetic relationship between BR6ox2 and selected P450 genes. BR6ox2 belongs to a group consisting of Dwarf (tomato, CYP85A1), BR6ox1 (Arabidopsis, CYP85A1), CPD (Arabidopsis, CYP90A1 and X87367), ROT3 (Arabidopsis, CYP90C1 and AB008097), DWF4 (Arabidopsis, CYP90B1 and AF044216), and CYP90D (Arabidopsis, AB066286). BAS1/CYP72B1 (Arabidopsis, BAS1 and AC003105) is reported to be involved in BR inactivation. Dwarf3 (maize [Zea mays], CYP88A and U32579) and GA3 (Arabidopsis, CYP701A and AF047720) are reported to be involved in GA biosynthesis. The first P450 genes functionally identified from higher plants are CYP73A (Helianthus annuus cinnamate 4-hydroxylase, Z17369) and CYP75A (Petunia hybrida flavonoid-3′,5′-hydroxylase, D14588); both belong to the higher plant-specific group A of P450 genes. The accession numbers of the other members are D30718 (CYP8), M93133 (CYP7A), and X90458 (CYP86A).